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KMID : 0545120020120050801
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 5 p.801 ~ p.806
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Quantitative Analysis of Leuconostoc mesenteroides and Lactobacillus plantarum Populations by a Competitive Polymerase Chain Reaction
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KOH, YOUNG-HO
KIM, MYOUNG-DONG/HAN, NAM SOO/SEO, JIN-HO
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Abstract
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A multiplex competitive polymerase chain reaction (PCR) method was developed for the rapid identification and quantification of Leuconostoc mesenteroides and Lactobacillus plantarum populations which are key microorganisms in kimchi fermentation. This strain-specific primers were designed to selectively amplify the target genes ensoding 16S rRNA of L. plantarum and dextransucrase of L. mesenteroides. There was a linear relationship between the band intensity of PCR products and the number of colony forming units of each model organism. The PCR quantification method was compared with a traditional plate-counting method for the enumeration of the two lactic acid bacteria in a mixed suspension culture and also applied to a real food system, namely, watery kimchi. The population dynamics of the two model organism in the mixed culture
were reliably predictably by the competitive PCR analysis.
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